Standard ez load and amplisize rulers.
Bio rad 20 bp ladder.
500 μl 0 05 μg μl ready to load dna standard 100 1 000 bp 10 bands includes 1 ml 5x nucleic acid sample buffer 100 applications.
Bio rad provides three types of molecular rulers.
We recommend loading 0 5 1 0 µg of 50 bp dna ladder diluted in sample buffer.
Standard ez load and amplisize rulers.
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250 μl 0 2 μg μl dna standard 20 1 000 bp 50 bands in 20 bp increments 100 applications.
Gel images were managed using the bionumerics software package version 4 0 applied maths sint.
If samples need to be diluted use te or other buffer of minimal ionic strength.
For long term storage store at 20 c.
Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Monitor electrophoresis and blotting experiments estimate molecular weights.
50 bp dna ladder is stable for at least 3 months at 4 c.
Use 1 0 5 0 µl for full length gels 16 20 cm to yield 100 or up to 500.
Bio rad molecular rulers are dna standards with precisely defined size intervals between bands dna ladders for simplified estimation of the length of nucleic acids separated on agarose gels.
Bio rad provides three types of molecular rulers.
Or 0 5 2 5 µl for silver staining up to 1 000 applications.
Dna may be denatured if diluted in dh 2 o.
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The size markers used were a 100 bp ladder ez load 100 pb pcr molecular ruler biorad marnes la coquette france or 20 bp ladder ez load 20 pb molecular ruler biorad marnes la coquette france according to the tandem repeat unit length.
1x gel loading dye purple no sds.